Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein‐α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice

Abstract Background Fibroblast activation protein‐α (FAP) and livin α are considered as cancer‐associated fibroblasts (CAFs) tumor‐specific targets, respectively, for immunogenic tumor vaccines. This study is designed to decipher the antitumor effect of double‐gene modified dendritic cells (DCs) on Lewis lung carcinoma (LLC). Methods By encoding mouse FAP cDNA human (i.e., hlivin α) into recombinant adenoviral vector (rAd), rAd‐FAP, rAd‐hlivin α, rAd‐FAP/hlivin were constructed, which then transduced DCs. LLC‐bearinig mice immunized with infected DCs (5 × 10 5 cells/mouse), followed by calculation volume survival rate. The identification CAFs from LLC well determination expressions was accomplished western blot. Cytotoxic T lymphocyte assay harnessed assess inducing splenic lymphocytes lyse CAFs. Results successfully in vitro. highly expressed positive α‐SMA negative CD45 CD31. Livin level upregulated LLC. Immunization α‐transduced suppressed improved tumor‐bearing mice. enhanced cytotoxic tumor‐derived Conclusion Injection promotes immune‐enhanced microenvironment decreasing suppresses growth models.